Formulation of Soil Beneficial Microbes Solid Inoculant for Controlling Nematode in Coffee

Plant Growth Promoting Rhizobacteria Pseudomonas and Bacillus are well known biofertilizer due to their ability for solubilizing inorganic phosphate producing phytohormones in soil. However, potential decreased diseases incidence caused by Pratylenchus coffeae has been documented. The objective of this laboratory experiment was formulate solid contained diminuta subtilis. experiments consisted three-step experiment: 1) selection the molasses-based liquid media P. B. subtilis growth, 2) organic inoculant formulation 3) carrier-based formulation. population both bacteria all steps count serial dilution plate method with specific medium. result verifeid that cell density culture 2% molasses were higher than 4% molasses. Bagasse-based carrier inoculated mixed bacterial compared manure-based carrier. After 90-day incubation, bagasse-based maintained up 108 CFU/mL acidity inculaant about neutral. agreed Indonesian standard